Stationary Phases for LC Separations at Elevated Temperatures

Please direct correpsondence to Yu Yang at [email protected]. With the advent of column materials that can be used effectively at temperatures well over 100 °C, high temperature liquid chromatography (HTLC) separations have gained more attention in the last few years. Advantages of HTLC include fast analysis time, better selectivity, improved column efficiency, and minimization of the use of organic solvents involved in the mobile phase. With the rapid heat transfer of packed capillary and open tubular columns, the use of temperature programming can supplement the use of gradient elution. Separation using high-temperature water as the sole eluent has shown a great potential as an alternative reversed-phase LC technique with the effect of having a “greener” mobile phase system. In this paper, silica-based, metal oxide-based, polymer, monolithic, and temperatureresponsive stationary phases have been reviewed. A recently developed high performance LC chip and other noteworthy aspects of HTLC also have been included in this review.